addgene 105677 aav1 chemicals Search Results


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addgene 105677 aav1 chemicals  (Addgene inc)
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paav hsyn1 sio stgtacr2  (Addgene inc)
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(A) Unilateral stimulation of VTAVgat+ neurons. AAV-DIO-ChR2 or <t>AAV-SIO-StGtACR2</t> was injected into the VTA of Vgat-ires-Cre mice. Inset shows ChR2 in the vicinity of TH-positive cells. Note glial scarring caused by optic fiber above VTA.
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(A) Unilateral stimulation of VTAVgat+ neurons. AAV-DIO-ChR2 or <t>AAV-SIO-StGtACR2</t> was injected into the VTA of Vgat-ires-Cre mice. Inset shows ChR2 in the vicinity of TH-positive cells. Note glial scarring caused by optic fiber above VTA.
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(A) Unilateral stimulation of VTAVgat+ neurons. AAV-DIO-ChR2 or <t>AAV-SIO-StGtACR2</t> was injected into the VTA of Vgat-ires-Cre mice. Inset shows ChR2 in the vicinity of TH-positive cells. Note glial scarring caused by optic fiber above VTA.
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aav1 cag tdtomato  (Addgene inc)
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(A) Unilateral stimulation of VTAVgat+ neurons. AAV-DIO-ChR2 or <t>AAV-SIO-StGtACR2</t> was injected into the VTA of Vgat-ires-Cre mice. Inset shows ChR2 in the vicinity of TH-positive cells. Note glial scarring caused by optic fiber above VTA.
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Image Search Results


(A) Unilateral stimulation of VTAVgat+ neurons. AAV-DIO-ChR2 or AAV-SIO-StGtACR2 was injected into the VTA of Vgat-ires-Cre mice. Inset shows ChR2 in the vicinity of TH-positive cells. Note glial scarring caused by optic fiber above VTA.

Journal: Current biology : CB

Article Title: Precise Coordination of Three-dimensional Rotational Kinematics by Ventral Tegmental Area GABAergic Neurons

doi: 10.1016/j.cub.2019.08.022

Figure Lengend Snippet: (A) Unilateral stimulation of VTAVgat+ neurons. AAV-DIO-ChR2 or AAV-SIO-StGtACR2 was injected into the VTA of Vgat-ires-Cre mice. Inset shows ChR2 in the vicinity of TH-positive cells. Note glial scarring caused by optic fiber above VTA.

Article Snippet: Method Details Viral Constructs rAAV5.EF1α.DIO.hChR2(H134R).eYFP and rAAV5.EF1α.DIO.eYFP were obtained from the Duke University Vector Core. pAAV-hSyn1-SIO-stGtACR2-FusionRed (Mahn et al., 2018) and pGP-AAV-syn-jGCaMP7f-WPRE (Dana et al., 2018) were obtained from Addgene. pGP-AAV-syn-jGCaMP7f-WPRE was from Douglas Kim (Addgene viral prep # 104488-AAV1; http://n2t.net/addgene:104488 ; RRID:Addgene_104488). pAAV_hSyn1-SIO-stGtACR2-FusionRed was from Ofer Yizhar (Addgene viral prep # 105677-AAV1; http://n2t.net/addgene:105677 ; RRID:Addgene_105677).

Techniques: Injection

Key Resources Table

Journal: Current biology : CB

Article Title: Precise Coordination of Three-dimensional Rotational Kinematics by Ventral Tegmental Area GABAergic Neurons

doi: 10.1016/j.cub.2019.08.022

Figure Lengend Snippet: Key Resources Table

Article Snippet: Method Details Viral Constructs rAAV5.EF1α.DIO.hChR2(H134R).eYFP and rAAV5.EF1α.DIO.eYFP were obtained from the Duke University Vector Core. pAAV-hSyn1-SIO-stGtACR2-FusionRed (Mahn et al., 2018) and pGP-AAV-syn-jGCaMP7f-WPRE (Dana et al., 2018) were obtained from Addgene. pGP-AAV-syn-jGCaMP7f-WPRE was from Douglas Kim (Addgene viral prep # 104488-AAV1; http://n2t.net/addgene:104488 ; RRID:Addgene_104488). pAAV_hSyn1-SIO-stGtACR2-FusionRed was from Ofer Yizhar (Addgene viral prep # 105677-AAV1; http://n2t.net/addgene:105677 ; RRID:Addgene_105677).

Techniques: Virus, Plasmid Preparation, Software